Glycolysis

Rate limiting step - phosphofructo kinase
Fluoride inhibits enolase
Lactate dehydrogenase converts pyruvate to lactic acid in anerobic resp.

ATP
Steps 1 and 3 = - 2ATP
Steps 6 and 9 = + 4 ATP
Net "visible" ATP produced = 2.


NADPH
Reaction 5 is an oxidation where NAD⁺ removes 2 hydrogens and 2 electrons to produce NADH and H⁺. Since this reaction occurs twice, 2 NAD⁺ coenzymes are used.

Step	ATP (used -) (produced +)
1	-1
3	-1
5 - NADH to E.T.C to FAD = 2 step 5 used twice	2 x 2 = +4
6 used twice	1 x 2 = + 2
9 used twice	1 x 2 = + 2
NET	6 ATP